ORITaq DNA Polymerase
#10222-001 500 U
#10222-005 5 × 500 U
ORITaq DNA Polymerase is purified from E. coli expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5´→3´ DNA polymerase and a 5´→3´ exonuclease activity but lacks a 3´→5´ exonuclease activity. Taq DNA polymerase is heat-stable and synthesizes DNA at elevated temperatures from single-stranded templates at the presence of primers. PCR products generated using ORITaq DNA Polymerase contain 3’dA overhangs, and can be used for downstream TA cloning.

10222-001
10222-005
ORITaq DNA Polymerase (5 U/μl)
100 μl
5 × 100 μl
10× ORITaq PCR Buffer*
1 ml
5 × 1 ml
*10× ORITaq PCR Buffer : 100 mM Tris-Cl (pH 8.3), 15 mM MgCl2, 500 mM KCl.
@ -20°C for 2 years. Avoid repeated freeze and thaw cycles.
• High purity recombinant Taq Polymerase
• Amplification of PCR products up to 5 kb
• Add Mg2+ in buffer for direct use
• Good sensitivity with high yield in amplification
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