2× ORITaq PCR Mix (with Loading Dye)
| #10224-001 | 0.5 ml | |
| #10224-005 | 5 × 0.5 ml |
OriBioTM 2× ORITaq PCR Mix (with Loading Dye) is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, and stabilizers. It is generally used at 1× concentration with DNA template and primers in a total reaction volume of 20 or 50 μl. 2× ORITaq PCR Mix has the benefits of quick and easy setup, high sensitivity, specificity, and good stability, minimizing manual error, time saving, contamination reduction, and is ideal for general PCR reactions, high-throughput screening, and other routine applications. The amplification products generated using 2× ORITaq PCR Mix contain dA overhangs at the 3´ends, and are suitable for downstream TA cloning. The dye-containing end products after PCR reaction can be loaded for gel electrophoresis and detected directly, and can be purified for subsequent experiments. The red dye contained migrates at a rate that is close to a double-stranded DNA fragment of 300 bp in a 1% TAE agarose gel.
Routine PCR, Primer Extension, Microarray, High-Throughput PCR
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|
10224-001 |
10224-005 |
|
2× ORITaq PCR Mix(with Loading Dye) |
0.5 ml |
5 × 0.5 ml |
|
ddH2O |
0.5 ml |
5 × 0.5 ml |
@ -20°C for 2 years. Avoid repeated freeze and thaw cycles.
• Good sensitivity with high yield
• Amplification of PCR products up to 5 kb
• Ease of use with minimal steps
• Time-saving and less error
• Analyzing PCR products without additional steps
• Amplification of PCR products up to 5 kb
• Ease of use with minimal steps
• Time-saving and less error
• Analyzing PCR products without additional steps